For Faster & Expansive Extraction

Buffalo Extraction Systems’ Level 2 and 3 Carbon dioxide Supercritical Fluid Extraction (CO2 SCFE) systems cater to medium and large scale extraction operations. As compared to Level 1 systems, these have larger extractor capacities and automated operation of the extractor changeover valves [1].

Operator convenience is an integral part of these ergonomically designed systems that come with an intuitive user interface. Safety automation, rapidly opening closures, and automatically working extractor changeover valves guarantee highest possible productivity with minimal downtime [1].


Level 2 and Level 3 CO2 SCFE System: Technical Specifications

Ergonomics is built into the Level 2 and 3 systems making it simple and comfortable for personnel to handle them. An intuitive user interface provides a single interface operation to monitor the values of all the parameters in the system. Personnel can supervise the process at all times.

Table 1 lists out the technical specifications of the Level 2 and 3 Carbon dioxide (CO2) Supercritical Fluid Extraction (SCFE) systems.



Parameter Level 2 Specification Level 3 Specification
Volume of Single Extractor 25 / 50 Litre 100 / 200 / 500 Litre
Number of Extractors 2 / 3 2 / 3
Usage of Extractors Single / Series Single / Series
Pressure 350-650 Bar / 5000-9000 PSI 350-650 Bar / 5000-9000 PSI
Temperature 70-110 0C / 158-230 0F 70-110 0C / 158-230 0F
Extractor Changeover Valves Automatic Automatic
Flow Rate of CO2 Pump 40-80 LPH 40-80 LPH
CO2 Recirculation Yes Yes
CO2 Feeding & Recovery System    Included Included
Co-Solvent Pump Yes Yes
Certifications CE / U1 / U2 / cGMP


CE / U1 / U2 / cGMP


Table 1. Technical Specifications of Buffalo Extraction Systems’ Level 2 and 3 Supercritical Fluid Extraction (SCFE) System [2]


  • Capacity, Number, and Arrangement of Extractors: For the Level 2 system, extractor volume can either be 25 litres or 50 litres, while the Level 3 system comes with extractor volumes in three options – 100 lit, 200 lit, or 500 lit [2]. Number of deployable extractors for both systems varies between two and three, and these can be arranged in isolation or in series. For Level 3 systems, further customized extractor capacities are also possible to cater to specific production needs. A noteworthy feature of these systems is the quick opening closures and safety automation which allow operators to execute swift changeovers [1]. Such fast operation means the system continues functioning with minimal interruptions even during batch processing, for maximum productivity. Make of the extractors is based on the directives provided under ASME, Chapter VIII that deals with the design, manufacture, inspection, and testing of pressure vessels [3]. Additionally, U-Stamp Certification is provided as per geographical requirement.


  • Extractor Pressure: These systems are offered as those with maximum extraction pressure of 350 bar (5,000 PSI) or 650 bar (9,000 PSI) [2]. Extractor pressure is a crucial parameter and the wide range enables the Level 2 and 3 systems to extract different target molecules from various raw stock.


  • CO2 Pump Flow Rate: Influences the yield [4], efficiency [5], and mass transfer rate [6] of the CO2 SCFE process and, as such, is a critical parameter. Varying the flow rate changes the resident time over which the solvent acts on the raw material. Flow rates are, therefore, optimized to ensure favourable extraction results.
    Level 2 and 3 systems employ a proprietary CO2 plunger pump to maintain the required flow rate at a constant value. High pressure plunger pumps blend in well with SCFE systems for the dietary supplement, pharma, food, and cosmetic sectors [7].


  • Automation: Proprietary extractor changeover valves can be rapidly and automatically used to ensure quick changeover between extractors. This allows the Level 2 and 3 systems to function with minimum downtime.


  • Temperature: Level 2 and 3 systems can have max operating temperatures of up to 70 0C (158 0F) or up to 110 0C (230 0F) [2]. Apart from pressure, temperature too influences the density of the solvent (supercritical CO2), which, in turn, impacts its solvent power.


  • Available Certifications: Buffalo Extraction Systems purpose builds Level 2 and 3 systems as per the existing Good Manufacturing Practice (GMP) standards. Such hygienic and customized design complies with the relevant food safety directives and factors in the actual conditions on the client’s shop floor. The location and operation of the related components (drain valves for example) is such that they are easy to access and clean with standard CIP systems [1].


  • Co-Solvent Pump: Adds co-solvents to supercritical carbon dioxide (sCO2) in the necessary quantity. These improve the capacity of sCO2 to dissolve non-polar ingredients. On its own, sCO2 is an excellent solvent for polar compounds. Co-solvents include ethanol, water, and methanol [8].


  • CO2 Recirculation & CO2 Feeding-Recovery System: Recovering the CO2 and re-circulating it through the systems means the gas does not escape to the atmosphere where it can produce the unwanted greenhouse effect. Moreover, recovery minimizes operational expenditure by decreasing the amount of fresh gas fed into the system.




Incorporating innovation through close process parameter control, superior ergonomics, rapid changeover mechanisms, and ingrained focus on safety, Buffalo Extraction Systems lends an innovative dimension to the already fantastic proficiency of carbon dioxide supercritical fluid extraction.


Buffalo Extraction Systems amplifies the great capabilities of the Carbon dioxide Supercritical Fluid Extraction process and places fantastic deliverables at your disposal.

Write to us at and get a first hand feel of genuine innovation at work.




  1. Level 2/3. Cybernetik Technologies Extraction.
  2. Our Systems. Cybernetik Technologies Extraction.
  3. ASME Code Section 8. Inspection 4 Industry LLC.  
  4. Effect of Solvent Flow Rate on the Supercritical Carbon Dioxide Extraction of Andrographolide from Andrographis Paniculata. A.C. Kumoro et al. Core.  
  5. Recent Advances in Lipid Extraction for Biodiesel Production. Narges Moradi-kheibari et al. Advances in Feedstock Conversion Technologies for Alternative Fuels and Bioproducts. Science Direct.  
  6. Effect of Pressure, Temperature and Flow Rate on Supercritical Carbon dioxide Extraction of Bottle Gourd Seed Oil. Prashant Said et al. International Journal of Food and Nutritional Sciences. Research Gate.  
  7. Oscillating Displacement Pumps – Sustainable Extraction with Carbon Dioxide. Michael Lenz et al. Uraca.  
  8. Why and How are Co-solvents Sometimes Used in the SFE Process? Supercritical Fluid Technologies, Inc.